Many groups have contributed to the vitality of the island by their active use of the island and buildings. The Orono Land Trust has built and maintains walking paths, The Orono Historical Society has held meetings on the island and has been a baluable resource by providing historical photographs. The island has been used by the Orono Fire and Police Deparments for training and Ayers Island, LLC has donated space for the headquarters of Dirigo Search and Rescue.

Below is a list of public organizations that have used Ayers Island for varying activities:

Orono Land Trust
Orono Fire Department
River Keepers
Dirigo Search and Rescue
Orono Historical Society
The National Gaurd
Orono Police Department